Journal: bioRxiv
Article Title: Porosome reconstitution therapy: A biologic rescue from cystic fibrosis
doi: 10.1101/2024.09.11.612494
Figure Lengend Snippet: (A) Wide-field fluorescence images of untreated HBE cells (left) and HBE cells with CFTR ΔF508 mutation treated with increasing concentrations of porosomes (0.5, and 1 µg). The scale bar represents 50 µm. DAPI (blue) stains the nuclei, and CFTR (green) highlights the CFTR protein. ( B) Magnify-processed images of the same conditions as in Panel A, showing enhanced resolution and detail of CFTR expression. The scale bar represents 50 µm. ( C) Zoomed-in images of the areas indicated by yellow-dotted boxes in Panel B. These images provide a closer view of CFTR expression in the HBE cells. The scale bar represents 5 µm. (D) Magnify-processed images of CFTR ΔF508-mutated HBE cells treated with increasing doses of porosomes (0.2, 0.5, and 1 µg). This panel shows DAPI (blue), CFTR (green), and SNAP23 (red), illustrating the expression and localization of both proteins in response to porosome treatment. The scale bar represents 5 µm in biological scale. B, C and D: The expansion factor is 3.8× (1× PBS).
Article Snippet: Cells were incubated with a staining buffer (2X SSC, 0.2% Tween-20, 1X PBS) containing primary antibodies (1:500 dilution): mouse anti-CFTR (Invitrogen; MA1-935) and rabbit anti-SNAP23 (Invitrogen; PA5-28936) for 45 minutes to 1 hour at room temperature.
Techniques: Fluorescence, Mutagenesis, Expressing